Nonfimbrial, mannose-resistant adhesins from uropathogenic Escherichia coli O83:K1:H4 and O14:K?:H11

Screening of uropathogenic Escherichia coli for expression of mannose-selective adhesins: importance of culture conditions.

I surveyed the prevalence of mannose-sensitive adherence factors (adhesins) in uropathogenic Escherichia coli isolates by using several rapid screening tests based on the agglutination of stained yeast cells. Mannose-sensitive adhesin activity was demonstrated best for bacteria grown in aerobic static broth cultures. Under these conditions, the majority of the strains from patients with signifi...

The role of mannose sensitive and mannose resistant pili of Escherichia coli in urinary tract infection

  In order to study the relationship between hrmolysin / ashesins of Escherichia coli and occurrence of urinary tract infection ( UTI), the capacity of 570 isolated E.coli in agglutination of human or guinea pig erythrocytes and their lysis were tested. These isolates were obtained from the urine of patients with bacteriuria and from the stool of healthy people. Three patterns of hemagglutinat...

Comparative structure-function analysis of mannose-specific FimH adhesins from Klebsiella pneumoniae and Escherichia coli.

FimH, the adhesive subunit of type 1 fimbriae expressed by many enterobacteria, mediates mannose-sensitive binding to target host cells. At the same time, fine receptor-structural specificities of FimH from different species can be substantially different, affecting bacterial tissue tropism and, as a result, the role of the particular fimbriae in pathogenesis. In this study, we compared functio...

uropathogen . nonfimbrial adhesin I from an Escherichia coli

Dimeric and Trimeric Fusion Proteins Generated with Fimbrial Adhesins of Uropathogenic Escherichia coli

Urinary tract infections (UTIs) are associated with high rates of morbidity and mortality worldwide, and uropathogenic Escherichia coli (UPEC) is the main etiologic agent. Fimbriae assembled on the bacterial surface are essential for adhesion to the urinary tract epithelium. In this study, the FimH, CsgA, and PapG adhesins were fused to generate biomolecules for use as potential target vaccines...